Analysis of X-ray diffraction fingerprint of Calamina and its processed products / 中草药

Objective: To establish X-ray diffraction (XRD) fingerprint of Calamina and its processed products, compare the effects of different processing Methods on the main components of medicinal materials and determine the content of ZnO in the processed products. Methods: XRD was used to analyze 10 batches of Calamina and its processed products, and fingerprints of Calamina and its processed products were established respectively. Six different processing methods were compared, and the content of ZnO in all processed products was determined by K value method. Results: Fingerprints of Calamina and its processed products were preliminarily established. There were 23 common peaks in the fingerprints of Calamina, and there were 10 common peaks in the fingerprints of its processed products. After calcination, the ZnCO3 characteristic peak of the raw material was transformed into the characteristic peak of ZnO; The content of ZnO in the calcined product exceeded 56%. Conclusion: XRD fingerprints could be used for the identification and analysis of Calamina and its processed products. The new and reliable method was provided for quality evaluation of Calamina and its processed products.

Effect of different sandblasting conditions on the metal-ceramic bonding strength of Co-Cr alloy fabricated by selective laser melting technology / 华西口腔医学杂志

OBJECTIVE@#To investigate the effect of different sandblasting conditions on the metal-ceramic bonding strength of Co-Cr alloy fabricated by selective laser melting (SLM) technology.@*METHODS@#A total of 63 specimens of Co-Cr alloy fabricated by SLM were prepared and randomly divided into nine groups (n=7). Each group was treated with different powder particles (A1=50 µm, A2=100 µm, and A3=150 µm) and pressures (B1=0.2 MPa, B2=0.4 MPa, and B3=0.6 MPa) in sandblasting. One sample was randomly selected from each group for microstructure observation by scanning electron microscope (SEM). Ceramic was fired at the center of the specimens. Metal-ceramic bonding strength was measured with universal testing machine. Results were statistically analyzed with SPSS 17.0 software.@*RESULTS@#The mean bond strengths were as follows: Group A1B1: 27.22 MPa±0.95 MPa, Group A1B2: 27.58 MPa±0.47 MPa, Group A1B3: 26.80 MPa±0.71 MPa, Group A2B1: 27.54 MPa±0.78 MPa, Group A2B2: 30.75 MPa±0.43 MPa, Group A2B3: 26.93 MPa±0.88 MPa, Group A3B1: 28.18 MPa±0.93 MPa, Group A3B2: 29.55 MPa±0.57 MPa, and Group A3B3: 28.11 MPa±0.91 MPa. The particle factor of Al₂O₃ and the pressure factor of blasting showed statistical significance (P<0.05). An interaction was observed between the factors of particle and pressure (P<0.05). Mixed fracture mode of all specimens was observed after the shear strength test.@*CONCLUSIONS@#In conclusion, metal-ceramic bonding strength reaches the maximum when specimens are sandblasted with 100 µm alumina oxide at 0.4 MPa pressure.

Genetic variants in CHEK1 gene are associated with the prognosis of thoracic esophageal squamous cell carcinoma patients treated with radical resection / 华中科技大学学报（医学）（英德文版）

CHEK1 gene is known to play an important role in tumor progression by cell cycle control. However, the association between CHEK1 and the prognosis of esophageal squamous cell carcinoma (ESCC) is unclear. In this study, we explored the association between genetic variants in CHEK1 gene and prognosis of ESCC patients treated with radical resection. A total of 131 thoracic ESCC patients who underwent radical resection were included in this retrospective study and genotyped using the MassArray method. According to the univariate Cox hazard analysis, the GT/TT genotype of CHEK1 rs555752 was shown to be strongly related to a decreased overall survival (OS) (HR=2.560, 95% CI: 1.415-4.631, P=0.002) and disease-free survival (DFS) (HR=2.160, 95% CI: 1.258-3.710, P=0.005). Furthermore, according to the multivariate Cox hazard analysis and multiple testing, patients with the GT/TT genotype of CHEK1 rs555752 had a notably decreased OS (HR=2.735, 95% CI: 1.468-5.096, P=0.002, Pc=0.006) and DFS (HR=2.282, 95% CI: 1.292-4.023, P=0.004, Pc=0.012). In conclusion, genetic variants of the CHEK1 gene are significantly related to OS and DFS of ESCC patients, and may therefore be predictors of the prognosis of thoracic ESCC after surgery.

Difference analysis of proteome between diabetic cataract and age related cataract / 中华实验眼科杂志

Background With the changes of diet and living style,the diabetes has become the major diseases affecting human health.Diabetic cataract is a common complication of diabetes. Objective The present study was to investigate the difference of lens proteomics between diabetic cataract and age related cataract using two dimensional electrophoresis (2-DE) and mass spectrometry in order to postpone happening of diabetic cataract and offer the effective approach to the prevention and therapy of diabetic cataract. Methods The lenses were obtained from 8 diabetic patients and 12 age-related cataract patients during the surgery to extract the protein by lysis and centrifugation.The lens proteins were separated using immobilized pH gradients 2-DE.Image analysis was carried out using PDQuest Advanced-8.0.1 software package.Significant difference of the crystallines was identified by matrixassisted laser adsorption/ionization time of-flight-mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprint combined with protein database. Results The maps of 2-DE showed that lens proteins of diabetic cataract and age related cataract were in the section of pH 5-9 with the relative molecular weight 14000-97000;while relative molecular weight of more abundant crystalline was localized at 20000-31000.About 3 differential protein spots were detected by image analysis software.Two crystallines,αB and βB1 crystallin,were identified using MALDI-TOF-MS.Conclusions Proteomic analysis of lens can be accomplished and the proteins can be well separated,moreover,differential proteins can be analyzed using 2-DE and mass spectrometry between diabetic cataract and age related cataract.These results indicate that αB and βB1 crystallin proteins accelerate the development of diabetic cataract.This technique offers a new avenue for clarity of lens proteins of diabetic cataract other than age related cataract.